@article{YKXB3753,
author = {Xia Zhang 和 Jiaolian Liu 和 Siqi Xiong 和 Xiaobo Xia 和 Huizhuo Xu},
title = {Expression of Netrin-1 in Diabetic Rat Retina},
journal = {眼科学报},
volume = {28},
number = {3},
year = {2016},
keywords = {},
abstract = {Purpose:.To detect the expression of Netrin-1 in the retinas of diabetic rats and evaluate the relationship between Netrin-1mRNA and diabetic retinopathy(DR).Methods:.Twenty healthy adult male Sprague-Dawley(SD)rats were randomly divided into a diabetic model group(DM)and a normal control group(NC), each group was composed of ten rats..Sreptozocin(STZ)was administered intraperitoneally at a single dose of 60 mg / kg to diabetic group. The control rats were injected only with citrate buffer. Collection of serum at 72 h after STZ treatment and measuring blood glucose levels confirmed the development of diabetes..The rats with blood glucose level 16.67 mmol / L or higher were considered to be diabetic and were used in the experiment. Retinal tissues were harvested and expression of netrin-1 mRNA and protein in the retina tissues was examined by reverse transcription polymerase chain reaction(RT-PCR) and western blot analysis, respectively.Results:.Diabetic rats showed classic symptoms of diabetes mellitus,.such as polydipsia,.polyphagia,.and polyuria.Cataract was seen in the DM group at 3 months after administration of STZ. Both netrin-1 mRNA and protein levels retina were dramatically increased in the DM rats compared to the NC group(P},
url = {https://ykxb.amegroups.com/article/view/3753}
}