Effect of Avastin on the migration and invasion of pterygium fibroblasts
摘要
Purpose:.To evaluate the effect of Avastin on human pterygium fibroblast migration and invasiveness.Methods:.VEGF secretion was compared between human pterygium fibroblasts and conjunctival fibroblasts by measuring VEGF-A by ELISA. The influence of Avastin on HPF migration and invasiveness was observed by wound scratch and Transwell migration assays..The expression of p-ERK1 / 2 and p-FAK was analyzed by western blotting.Results:(1)VEGF was secreted in higher amounts by human pterygium fibroblasts than by conjunctival fibroblasts.(2)Avastin treatment decreased HPF migration and invasion.(3)Avastin significantly decreased the expression of p-ERK1 / 2and p-FAK in human pterygium fibroblasts.Conclusion:.Avastin can inhibit migration and invasion of HPFs by decreasing the expression of p-ERK1 / 2 and p-FAK.
